Differential Effects of Surfactants on Enzyme Activity and Transport across a Semipermeable Cell
نویسنده
چکیده
This study systematically investigated how anionic surfactants of various hydrophilicities affected the activities of three metabolically important enzymes –– glutamate dehydrogenase (GDH), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) –– of different molecular masses at a pH range important to body functions (6.5-7.4). We also investigated the time course of the surfactant concentration dependent effects on LDH. To observe how surfactants would affect the cellar protein transport, a separation cell with a semipermeable membrane was used to simulate a passive mass transport phenomenon between cells, with various concentration combinations of enzymes and surfactants.
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